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News from the lab

The Experimental Laboratory of Intensive Medicine has been one of the core platforms of the Biomedical Centre since 2004. It represents a comprehensively equipped experimental workplace faithfully simulating the intensive care environment, including the replacement of organ functions. The main long-term research goal is to elucidate the mechanisms of multi-organ failure in sepsis and septic shock with the identification and testing of new therapeutic approaches and targets, using clinically relevant animal experiments with translational potential and real impact on clinical practice. In doing so, we have long-standing collaborations with antibiotic resistance and experimental cardiology laboratories.

Long-term research objectives

  • Elucidation of the etiopathogenesis of acute (multi)organ damage in critical conditions, especially in septic shock.
  • Identification and testing of new therapeutic targets and molecules in the treatment of life-threatening infections.
  • Testing the efficacy, safety and mechanism analysis of new methods of organ replacement and support.

Current research

In the current year, we are continuing a long-term project investigating the effect of our engineered molecule containing an anti-sense oligonucleotide targeting the bacterial wall synthesis gene and the effect of this intervention in modulating virulence and antibiotic resistance in sepsis and septic shock. The research builds on a pilot study conducted last year and is scheduled for completion in the fall of 2025.

Team

prof. MUDr. Martin Matějovič, Ph.D. – vedoucí výzkumného programu 
prof. MUDr. Jan Beneš, Ph.D. 
MUDr. Jiří Müller – vedoucí laboratoře 
MUDr. Mgr. Jan Horák, PhD. 
MUDr. Václav Tégl
MUDr. Lukáš Nalos, Ph.D.
MUDr. Pavel Vintrych
Bc. Hanzlíková Miroslava
Bc. Jánská Karin

Publications and cooperation

Major publications and collaborations of the laboratory over the years
2024Tegl, V. et al. (2024) “Ineffectiveness of hemoadsorption in large animals with abdominal sepsis: a randomized controlled porcine study,” Intensive care medicine experimental https://doi.org/10.1186/s40635-024-00622-x
2023Vintrych, P. et al. (2023) “Modeling sepsis, with a special focus on large animal models of porcine peritonitis and bacteremia,” Frontiers in physiology https://doi.org/10.3389/fphys.2022.1094199
2022Chalupova, M. et al. (2022) “Gut microbiome diversity of porcine peritonitis model of sepsis,” Scientific reports https://doi.org/10.1038/s41598-022-21079-6
2020Chvojka, J. et al. (2020) “Mechanical circulatory support in refractory vasodilatory septic shock: A randomized controlled porcine study,” Shock https://doi.org/10.1097/shk.0000000000001329

Horak, J. et al. (2020) “Evaluation of mesenchymal stem cell therapy for sepsis: A randomized controlled porcine study,” Frontiers in imunology https://doi.org/10.3389/fimmu.2020.00126
2019Kohoutova, M. et al. (2019) “Vagus nerve stimulation attenuates multiple organ dysfunction in resuscitated porcine progressive sepsis,” Critical care medicine https://doi.org/10.1097/ccm.0000000000003714
2018Jarkovska, D. et al. (2018) “Cellular mechanisms of myocardial depression in porcine septic shock,” Frontiers in physiology https://doi.org/10.3389/fphys.2018.00726
2017Matejovic, M. et al. (2017) “Molecular differences in susceptibility of the kidney to sepsis-induced kidney injury,” BMC nephrology https://doi.org/10.1186/s12882-017-0602-x

Jarkovska, D. et al. (2017) “Heart-rate variability depression in porcine peritonitis-induced sepsis without organ failure,” Experimental biology and medicine https://doi.org/10.1177/1535370217700521
2016Jarkovska, D. et al. (2016) “Heart rate variability in porcine progressive peritonitis-induced sepsis,” Frontiers in physiology https://doi.org/10.3389/fphys.2015.00412

Matejovic, M. et al. (2016) “Renal proteomic responses to severe sepsis and surgical trauma: Dynamic analysis of porcine tissue biopsies,” Shock https://doi.org/10.1097/shk.0000000000000613

Tuma, Z. et al. (2016) “Mitochondrial proteomes of porcine kidney cortex and medulla: foundation for translational proteomics,” Clinical and experimental nephrology https://doi.org/10.1007/s10157-015-1135-x
2013Stengl, M. et al. (2013) “Effects of clinically relevant acute hypercapnic and metabolic acidosis on the cardiovascular system: an experimental porcine study,” Critical care https://doi.org/10.1186/cc13173
2011Benes, J. et al. (2011) “Searching for mechanisms that matter in early septic acute kidney injury: an experimental study,” Critical care https://doi.org/10.1186/cc10517

Krouzecky, A. et al. (2011) “The safety and efficacy of a new anticoagulation strategy using selective in-circuit blood cooling during haemofiltration–an experimental study,” Nephrology, dialysis, transplantation: official publication of the European Dialysis and Transplant Association – European Renal Association https://doi.org/10.1093/ndt/gfq622

Kuncová, J. et al. (2011) “Plasma and tissue levels of neuropeptide Y in experimental septic shock: Relation to hemodynamics, inflammation, oxidative stress, and hemofiltration,” Artificial organs https://doi.org/10.1111/j.1525-1594.2010.01154.x

Kuncová, J. et al. (2011) “Tissue concentrations of vasoactive intestinal peptide are affected by peritonitis-induced sepsis and hemofiltration in pigs,” Physiological research https://doi.org/10.33549/physiolres.932051
2010Stengl, M. et al. (2010) “Differential effects of hemofiltration and of coupled plasma filtration adsorption on cardiac repolarization in pigs with hyperdynamic septic shock,” Shock https://doi.org/10.1097/shk.0b013e3181ab6359

Stengl, M. et al. (2010) “Reduced L-type calcium current in ventricular myocytes from pigs with hyperdynamic septic shock,” Critical care medicine https://doi.org/10.1097/ccm.0b013e3181cb0f61
2009Sykora, R. et al. (2009) “Coupled plasma filtration adsorption in experimental peritonitis-induced septic shock,” Shock https://doi.org/10.1097/shk.0b013e318188dec5

Sykora, R. et al. (2009) “High versus standard-volume haemofiltration in hyperdynamic porcine peritonitis: effects beyond haemodynamics?,” Intensive care medicine https://doi.org/10.1007/s00134-008-1318-y

Krouzecky, A. et al. (2009) “Regional cooling of the extracorporeal blood circuit: a novel anticoagulation approach for renal replacement therapy?,” Intensive care medicine https://doi.org/10.1007/s00134-008-1271-9
2008Stengl, M. et al. (2008) “Continuous hemofiltration in pigs with hyperdynamic septic shock affects cardiac repolarization,” Critical care medicine https://doi.org/10.1097/ccm.0b013e31818f9eda

Chvojka, J. et al. (2008) “Renal haemodynamic, microcirculatory, metabolic and histopathological responses to peritonitis-induced septic shock in pigs,” Critical care https://doi.org/10.1186/cc7164
2007Matejovic, M. et al. (2007) “Effects of combining inducible nitric oxide synthase inhibitor and radical scavenger during porcine bacteremia,” Shock https://doi.org/10.1097/01.shk.0000235088.53421.6f
2006Kroužecký, A. et al. (2006) “Perfusion pressure manipulation in porcine sepsis: effects on intestinal hemodynamics,” Physiological research https://doi.org/10.33549/physiolres.930821
2005Matejovic, M. et al. (2005) “Effects of tempol, a free radical scavenger, on long-term hyperdynamic porcine bacteremia,” Critical care medicine https://doi.org/10.1097/01.ccm.0000162927.94753.63
2004Matejovic, M. et al. (2004) “Selective inducible nitric oxide synthase inhibition during long-term hyperdynamic porcine bacteremia,” Shock https://doi.org/10.1097/00024382-200405000-00010

Contact:

MUDr. Jiří Müller
T: 377 103 165
E: mullerji@fnplzen.cz